KMID : 0861020190340050021
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Korea Journal of Herbology 2019 Volume.34 No. 5 p.21 ~ p.28
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The effects of Socheongryong-Tang on LPS-induced lung inflammation rats model
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Jin Bo-Ram
Choi In-Young Hwanf Do-Young Ham Seong-Ho An Hyo-Jin
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Abstract
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Objectives : In present study, we investigated a therapeutic effect and optimum dose of Socheongryong-Tang (SCT) on LPS-induced lung inflammation rats model.
Methods : Male Sprague-Dawley rats (260 ¡¾ 10 g) were divided into 12 groups : Group 1 included the normal rats, and Group 2-12 were administrated LPS by intranasal injection to induce experimental lung inflammation. After 1 day of LPS administration, Group 3-9 were treated with SCT ¡¿ 1/4, ¡¿ 1/2, ¡¿ 1, ¡¿ 3, ¡¿ 6, ¡¿ 12 or ¡¿ 18, respectively. Group 10-12 (positive control) were treated with dexamethasone 1 §·/§¸ or acetylcystein 1.5 §·/§¸ or diclofenac?sodium 0.4 §·/§¸, respectively. After sacrifice, bronchoalveolar lavage fluid (BALF) was isolated. The levels of IL-1¥â, TNF-¥á, mucin glycoprotein 5AC (MUG5AC) were measured in BALF using enzyme-linked immunosorbent assay (ELISA).
Results : LPS injected rats exhibited outstanding lung inflammation manifestations, including increased amount of total cells and neutrophil, and upregulated inflammatory cytokines level in BALF. However, the administration of SCT ¡¿ 1/4, ¡¿ 1/2 and ¡¿ 1 decreased total cells and neutrophil, and suppressed the production of inflammatory cytokines, including IL-1¥â and TNF-¥á, and MUG5AC in BALF. Notably, inhibitory effect of SCT ¡¿ 1/2 and ¡¿ 1 on the level of TNF-¥á was markedly better than that of positive controls, dexamethasone and acetylcystein.
Conclusions : Taken together, these results suggest that SCT ¡¿ 1/2 and ¡¿ 1 has therapeutic effects on LPS- induced lung inflammation rats model.
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KEYWORD
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Socheongryong-Tang, LPS-induced lung inflammation rats model, SD rats
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